What You’ll Learn
1. Fundamentals of Sterile Technique
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Lab Setup & Hygiene: Design a mini-lab or home workspace; maintain aseptic conditions with pressure cookers, laminar flow hoods, and proper disinfectants.
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Media Preparation: Formulate and sterilize agar, grain, sawdust, and liquid media with precise moisture and pH control.
2. Tissue Culture & Spore Work
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Tissue Culture Technology: Excise clean mushroom tissue; initiate pure mycelium cultures on agar plates.
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Spore Technology: Collect and germinate spores; develop stable monokaryotic strains for breeding and experimentation.
3. Solid & Liquid Cultures
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Solid Culture: Scale from petri dishes to test tubes and jars; achieve rapid, uniform colonization.
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Liquid Culture: Prepare nutrient broths; use magnetic stirring and incubators to produce high-density mycelial slurries.
4. Mother Spawn & Master Culture
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Mother Spawn: Transition from lab cultures to bulk grain; establish a reliable mother spawn bank for repeat use.
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Master Culture Maintenance: Store and rejuvenate cultures for long-term viability using cryopreservation and slant techniques.
5. Commercial Spawn Types
Learn specialized techniques for:
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Grain Spawn (wheat, sorghum, millet)
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Sawdust Spawn (hardwood and softwood blends)
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Wood Chip & Beechwood Spawn
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Liquid Mycelial Slurry for rapid inoculations
6. Variety-Specific Protocols
Advanced spawn recipes and conditions for:
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Oyster (Pleurotus spp.)
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Button (Agaricus bisporus)
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Milky (Calocybe indica)
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Shiitake (Lentinula edodes)
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Enoki (Flammulina velutipes)
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Lion’s Mane (Hericium erinaceus)
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Ganoderma (Reishi)
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Cordyceps militaris
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Maitake (Grifola frondosa)
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Wood Ear (Auricularia spp.)
…plus guidance on collecting wild strains, strain isolation, and genetic preservation.
7. Scaling from Home to Industry
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Small-Scale Setup: Craft spawn in modest kitchen or garage labs using budget-friendly equipment.
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Commercial Scale: Plan and manage multi-chamber sterilization units, automated bag fillers, and large-capacity spawn rooms.
Hybrid Learning & Support
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Online Resources: Access pre-recorded video demos, comprehensive eBooks, and audio tutorials.
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Live Webinars & Lab Classes: Join weekly lab-based sessions for real-time demonstrations of key techniques.
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Doubt-Clearing Sessions: Participate in group Q&As every week, plus optional one-on-one troubleshooting classes.
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Exclusive Community Access: Network, share leads, and troubleshoot with peers in our dedicated WhatsApp and Facebook groups.
Who Should Join?
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Aspiring Mushroom Farmers seeking reliable spawn supplies.
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Agri-Entrepreneurs entering gourmet or medicinal mushroom markets.
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Hobbyists & Home Growers wanting to master sterile techniques and build a spawn-making side business.
Note: This course focuses solely on spawn production; it does not cover full-cycle mushroom cultivation.
